کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10976574 | 1108042 | 2012 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Short communication: Presence of G protein-coupled receptor 43 in rumen epithelium but not in the islets of Langerhans in cattle
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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![عکس صفحه اول مقاله: Short communication: Presence of G protein-coupled receptor 43 in rumen epithelium but not in the islets of Langerhans in cattle Short communication: Presence of G protein-coupled receptor 43 in rumen epithelium but not in the islets of Langerhans in cattle](/preview/png/10976574.png)
چکیده انگلیسی
Volatile fatty acids (VFA) are the major products of microbial fermentation in the rumen. Besides serving as substrates for energy generation, VFA are known to stimulate rumen development, increase serum insulin and glucagon concentrations, and regulate gene expression in cattle and sheep. The mechanisms underlying these regulatory effects of VFA are unknown, but the recent discovery that VFA can bind to G protein-coupled receptor 43 (GPR43) and 41 (GPR41) suggests that the regulatory effects of VFA may be mediated by these receptors. As a step toward testing this possibility, we determined whether GPR43 was expressed in bovine rumen wall and the pancreatic islets of Langerhans. Polyclonal antibody against a bovine GPR43 peptide was generated. The specificity of the antibody for bovine GPR43 was confirmed by Western blot analysis of recombinant bovine GPR43 protein. Immunohistochemical analyses using this antibody revealed the presence of GPR43-immunoreactive cells in the epithelium, but not in the other layers of cattle rumen wall. The same immunohistochemical analyses did not reveal GPR43-immunoreactive cells in the islets of Langerhans or the surrounding exocrine tissue of cattle pancreas. These data support the possibility that the effect of VFA on rumen epithelial growth in cattle is directly mediated by GPR43 in the rumen epithelial cells and that the effect of VFA on pancreatic secretion of insulin and glucagon in cattle is unlikely to be directly mediated by GPR43.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 95, Issue 3, March 2012, Pages 1371-1375
Journal: Journal of Dairy Science - Volume 95, Issue 3, March 2012, Pages 1371-1375
نویسندگان
A. Wang, R.M. Akers, H. Jiang,