کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10978635 1108059 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Technical note: Identification of Prototheca species from bovine milk samples by PCR-single strand conformation polymorphism
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Technical note: Identification of Prototheca species from bovine milk samples by PCR-single strand conformation polymorphism
چکیده انگلیسی
We report the development of a PCR-single strand conformation polymorphism (SSCP) method to identify Prototheca spp. responsible for bovine mastitis: P. zopfii and P. blaschkeae. The method was set up using reference strains belonging to P. zopfii genotype 1, P. zopfii genotype 2, and P. blaschkeae as target species and P. stagnora, and P. ulmea as negative controls. The assay was applied on 50 isolates of Prototheca spp. isolated from bovine mastitic milk or bulk-tank milk samples, and all isolates were identified as P. zopfii genotype 2. We conclude that the described PCR-SSCP approach is accurate, inexpensive, and highly suitable for the identification of P. zopfii genotype 2 on field isolates but also directly on milk, if preceded by a specific DNA extraction method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 95, Issue 12, December 2012, Pages 6963-6968
نویسندگان
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