کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11007619 | 1543268 | 2018 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzyme production by a fungoid marine protist, Thraustochytrium striatum
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کلمات کلیدی
DDIyeast extract and peptoneYEPPNPBstandard mediumASWSTMMWCOEPSEPACMCPGADCMp-nitrophenyl butyrate - p-nitrofenyl butyrateartificial sea water - آب دریا مصنوعیdeionized distilled water - آب مقطر دیونیزه شدهExtracellular enzyme - آنزیم خارج سلولیEicosapentaenoic acid - اسید ایکوزاپنتانوئیکdocosahexaenoic acid - اسید داکوزاگزوائونیکPolygalacturonic acid - اسید پلی گلاکترونیکDHA - دوکوساهگزائنوئیک اسیدCatabolite repression - سرکوب کاتابولیتMolecular weight cutoff - قطع وزن مولکولیExtracellular polymeric substances - مواد پلیمری خارج سلولیmarine protist - پروتئین دریاییCarboxymethyl cellulose - کربوکسی متیل سلولز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
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چکیده انگلیسی
Thraustochytrium striatum is a fungoid marine protist and was shown to be a promising enzyme producer for potential industrial applications. This research aimed at studying extracellular enzymes secreted by T. striatum under different conditions with specific objectives to qualitatively identify enzymes, quantify the cell growth and enzyme production, correlate enzyme production with extracellular polymeric substances (EPS), and examine the induction of enzyme by polysaccharide substrates. The qualitative analysis showed that T. striatum can produce at least seven extracellular enzymes including lipase and six polysaccharases (i.e., amylase, CMCase, xylanase, chitinase, pectinase, and κ-carrageenase). The carbon and nitrogen concentrations and salinity significantly affected the kinetics of enzyme production. T. striatum produced decent amount of polysaccharases at all conditions, but negligible lipase. Amylase was the predominant enzyme and reached the highest activity of 750â¯U/L with glucoseâ¯=â¯30â¯g/L, nitrogen sourceâ¯=â¯6â¯g/L and salinityâ¯=â¯100% sea water. Enzymes appeared to correlate with the production and monosaccharide composition of EPS. Enzyme-specific polysaccharide substrates including starch, CMC, xylan, κ-carrageenan, pectin, and chitin did not induce the production of corresponding enzymes by T. striatum while carbon starvation condition resulted in comparable enzyme activities, which indicated that enzymes from T. striatum were constitutive.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Protistology - Volume 66, October 2018, Pages 136-148
Journal: European Journal of Protistology - Volume 66, October 2018, Pages 136-148
نویسندگان
Rui Xiao, Xiang Li, Yi Zheng,