Combined delivery of angiopoietin-1 gene and simvastatin mediated by anti-intercellular adhesion molecule-1 antibody-conjugated ternary nanoparticles for acute lung injury therapy

The ternary ICAM-NLC/Pro/Ang nanoparticles containing angiopoietin-1 gene, protamine and ICAM-NLC were prepared via charge interaction firstly. The ICAM-NLC/Pro/Ang exhibited ideal lung-targeted ability in lipopolysaccharide-induced ALI mice after i.v. administration, as well as the significant up-regulation of Ang-1 protein in lung tissue. The ICAM-NLC/Pro/Ang realized an effective attenuation of pulmonary inflammation via co-delivery of Ang-1 gene and simvastatin to the injured lung. Collectively, the ICAM-NLC/Pro/Ang may represent a promising candidate favoring the clinical ALI therapy.331