کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11020856 | 1715611 | 2018 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Use of halophilic aquaporin for preparation of biomimetic thin film composite membrane
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کلمات کلیدی
AquaporinsPMSFAQPmPDPLRTMCDOPCHalomonas elongata1,2-dioleoyl-sn-glycero-3-phosphocholine - 1،2-dioleoyl-sn-glycero-3-phosphocholinepsf - PSFaquaporin - آکواپورینBiomimetic membranes - غشای Biomimeticphenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدdesalination - نمکزدایی یا شیرین کردنPolysulfone - پلی سولفونTrimesoyl chloride - کلرید Trimesoyl
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A new aquaporin from a moderate halophile, Halomonas elongata, for fabrication of desalination membrane is introduced in this study. For activity comparison, H. elongata aqp gene and E. coli aqpZ gene were cloned into pGEX-4T-2 vector and expressed in E. coli BL21 cells. E. coli AqpZ (EcAqpZ) appeared as tetrameric form, whereas H. elongata aquaporin (HeAqp) was found mostly as monomers in SDS-PAGE analysis. Light scattering measurements showed that incorporation of aquaporins into the liposomes vesicles resulted in increase in water permeability. HeAqp and EcAqpZ were embedded in 200â¯nm DOPC liposomes and immobilized with m-phenylene-diamine (MPD) and trimesoyl chloride (TMC) salt rejection layer on polysulfone membrane (PSf). Water with 800â¯mM NaCl concentration was used for salt rejection experiments. Increasing aquaporin concentration resulted in increase in water permeability of Halomonas aquaporin embedded membranes. % salt rejection of HeAqp and EcAqp incorporated PSf membranes were increased by three fold as compared to control membrane. Salt rejection and water permeability of HeAqp embedded PSf membrane was three and 25 fold higher than that of control membrane, respectively. H. elongata aquaporin, a halophilic aquaporin, was shown to be a suitable candidate for preparation of desalination membranes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Membrane Science - Volume 568, 15 December 2018, Pages 105-112
Journal: Journal of Membrane Science - Volume 568, 15 December 2018, Pages 105-112
نویسندگان
Nur Gazale ÃalıcıoÄlu, Gazel Ãyküm Ãzdemir, AyÅenur Ãztürk, AyÅen Yıldız, Hilal Yılmaz, Pınar Ergenekon, Mustafa Erbakan, Elif Erhan, Melek Ãzkan,