کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11026035 1666434 2018 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparation and anticoagulant properties of heparin-like electrospun membranes from carboxymethyl chitosan and bacterial cellulose sulfate
ترجمه فارسی عنوان
خواص آماده سازی و ضد انعقاد غشاهای الکترو اسپن از نوع هپارین از کربوکسی متیل کیتوزان و سولفات سلولز باکتریایی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Heparin-like membranes (CPBS) with nanofibers (approximate diameters of 100-500 nm) were prepared through electrospinning of a blended solution of carboxymethyl chitosan nanoparticle (CMCN, diameters 483 nm) and poly (vinyl alcohol) (CMCN/PVA) onto the surface of modified bacterial cellulose sulfate (BCS) membranes. SEM images confirmed that the CMCN were stretched to nanofibers during electrospinning. The presence of BCS on the collector of electrospinning machine increased the spinnability of CMCN/PVA solution. FTIR and XPS measurement revealed that there were SO3−, COO−, and OH groups on the surface of CPBS membrane, expressing structural similarity to heparin. CPBS membranes maintained hydrophilicity and the glutaraldehyde crosslinked CPBS membrane was stable in water. The clotting time and platelet adhesion experiments expressed the anticoagulant properties of CPBS. The APTT, TT and PT of CPBS increased up to 116.0%, 189.8%, and 50% than those of the plasma, (67.4 s, 16.2 s, and 48.4 s, respectively). No platelets adhered onto the surface of CPBS. An inflammatory response was determined according to activation of the macrophages seeded onto the membranes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 120, Part B, December 2018, Pages 1396-1405
نویسندگان
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