کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11027383 | 1666299 | 2018 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimization and comparison of GC-FID and HPLC-ELSD methods for determination of lauric acid, mono-, di-, and trilaurins in modified coconut oil
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Modified coconut oil (MCO) obtained from the glycerolysis of virgin coconut oil (VCO) and glycerol under various conditions should have different amounts of bioactive fatty acids (FAs) and acylglycerols (AGs). Methods were developed to analyze lauric acid (LA), monolaurin (ML), dilaurin (DL), and trilaurin (TL) in MCO samples using gas chromatography - flame ionization detector (GC-FID) and high performance liquid chromatography - evaporative light scattering detector (HPLC-ELSD). The purpose of this research is to optimize and compare GC-FID and HPLC-ELSD methods for determination of LA, ML, DL, and TL in MCO samples. All the standard curves exhibited good linearity (R2â¯â¥â¯0.9995), except for that of LA analyzed by HPLC-ELSD (R2â¯=â¯0.9971). The limits of detection (LODs) and quantification (LOQs) were found to be in the range of 0.033-0.260â¯mg/ml and 0.099-0.789â¯mg/ml for the GC-FID method and 0.040-0.421â¯mg/ml and 0.122-1.277â¯mg/ml for the HPLC-ELSD method, respectively. The GC-FID method (LODâ¯â¤â¯0.033â¯mg/ml) was more sensitive than HPLC-ELSD method (LODâ¯â¤â¯0.421â¯mg/ml) and showed satisfactory recoveries for LA analysis while HPLC-ELSD method (LODâ¯â¤â¯0.040â¯mg/ml) was more sensitive than GC-FID method (LODâ¯â¤â¯0.260â¯mg/ml) and exhibited acceptable recoveries for TL analysis. Both methods were applied to determine the MCO samples produced under varied conditions for glycerolysis. The results revealed that the developed GC-FID method is suitable for the quantification of LA, ML, and DL while the developed HPLC-ELSD method is appropriate for the determination of ML, DL, and TL. Both developed GC-FID and HPLC-ELSD methods produced reproducible results for the determination of LA, ML, DL, and TL in MCO samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1099, 1 November 2018, Pages 110-116
Journal: Journal of Chromatography B - Volume 1099, 1 November 2018, Pages 110-116
نویسندگان
Juthaporn Ponphaiboon, Sontaya Limmatvapirat, Amornrut Chaidedgumjorn, Chutima Limmatvapirat,