کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11027440 | 1666296 | 2019 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation and identification of immunomodulatory selenium-containing peptides from selenium-enriched rice protein hydrolysates
ترجمه فارسی عنوان
جداسازی و شناسایی پپتید های حاوی سلنیوم ایمنی مشتق از هیدرولیز پروتئین برنج حاوی سلنیوم
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کلمات کلیدی
سلنیوم، برنج، هیدرولیزات، فعالیت ایمن سازی آمینو اسید،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The RAW264.7 cell model was employed to screen immunomodulatory selenium-containing peptides from selenium-enriched rice protein hydrolysates (SPHs). Moreover, the selenium-containing peptides of high-activity protein hydrolysates were purified by Sephadex G-25, and identified by reversed phase ultra performance liquid chromatography coupled with triple quadrupole time-of-flight mass spectrometry. The results showed that 25 peptide sequences containing selenomethionine (SeMet) information above 90% of probability confidence were found in a fraction of alcalase hydrolysates. SeMDPGQQ and TSeMMM of 100% probability confidence were speculated as two novel selenium-containing peptide sequences. The artificially synthesized peptide TSeMMM was subsequently verified by an excellent immunomodulatory activity at a concentration of 80â¯Î¼g/mL. In conclusion, the immunomodulatory activity of SPHs was correlated to SeMet sequence in the structure of selenium-containing peptides, and TSeMMM with a stronger immunomodulatory activity demonstrated potential as functional food additives for improving human health.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 275, 1 March 2019, Pages 696-702
Journal: Food Chemistry - Volume 275, 1 March 2019, Pages 696-702
نویسندگان
Yong Fang, Xin Pan, Ermin Zhao, Yi Shi, Xinchun Shen, Jian Wu, Fei Pei, Qiuhui Hu, Weifen Qiu,