کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11030916 | 1646120 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular identification and functional characterization of a novel glutamate transporter in yeast and plant mitochondria
ترجمه فارسی عنوان
شناسایی مولکولی و خصوصیات عملکردی یک مونوجۀ گلوتامات جدید در مخچه و میتوکندری گیاهی
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
چکیده انگلیسی
The genome of Saccharomyces cerevisiae encodes 35 members of the mitochondrial carrier family (MCF) and 58 MCF members are coded by the genome of Arabidopsis thaliana, most of which have been functionally characterized. Here two members of this family, Ymc2p from S. cerevisiae and BOU from Arabidopsis, have been thoroughly characterized. These proteins were overproduced in bacteria and reconstituted into liposomes. Their transport properties and kinetic parameters demonstrate that Ymc2p and BOU transport glutamate, and to a much lesser extent L-homocysteinesulfinate, but not other amino acids and many other tested metabolites. Transport catalyzed by both carriers was saturable, inhibited by mercuric chloride and dependent on the proton gradient across the proteoliposomal membrane. The growth phenotype of S. cerevisiae cells lacking the genes ymc2 and agc1, which encodes the only other S. cerevisiae carrier capable to transport glutamate besides aspartate, was fully complemented by expressing Ymc2p, Agc1p or BOU. Mitochondrial extracts derived from ymc2Îagc1Î cells, reconstituted into liposomes, exhibited no glutamate transport at variance with wild-type, ymc2Î and agc1Î cells, showing that S. cerevisiae cells grown in the presence of acetate do not contain additional mitochondrial transporters for glutamate besides Ymc2p and Agc1p. Furthermore, mitochondria isolated from wild-type, ymc2Î and agc1Î strains, but not from the double mutant ymc2Îagc1Î strain, swell in isosmotic ammonium glutamate showing that glutamate is transported by Ymc2p and Agc1p together with a H+. It is proposed that the function of Ymc2p and BOU is to transport glutamate across the mitochondrial inner membrane and thereby play a role in intermediary metabolism, C1 metabolism and mitochondrial protein synthesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1859, Issue 11, November 2018, Pages 1249-1258
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1859, Issue 11, November 2018, Pages 1249-1258
نویسندگان
Vito Porcelli, Angelo Vozza, Valeria Calcagnile, Ruggiero Gorgoglione, Roberto Arrigoni, Flavia Fontanesi, Carlo M.T. Marobbio, Alessandra Castegna, Ferdinando Palmieri, Luigi Palmieri,