Pt-induced crosslinks promote target enrichment and protection from serum nucleases

This work presents a novel click-based method to enrich Pt(II)-bound biomolecules. Pt(II)-bound DNA and proteins are readily isolated using an oligonucleotide hybridization strategy. Interestingly, Pt(II)-dependent and click-independent crosslinking of a DNA oligonucleotide to serum albumin was observed, and DNA crosslinked to protein is dramatically protected from serum nucleases.246