کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11033302 | 1622607 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Waste office paper: A potential feedstock for cellulase production by a novel strain Bacillus velezensis ASN1
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
علوم زمین و سیارات
مهندسی ژئوتکنیک و زمین شناسی مهندسی
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چکیده انگلیسی
This paper reports the cellulase (FPase) production by newly isolated Bacillus velezensis ASN1 using waste office paper (WOP) as feedstock and optimization of production conditions through two level factorial design, steepest ascent/descent and second order response surface methodology (RSM). Various fermentation parameters, like chemical factors (potassium dihydrogen phosphate, potassium chloride, yeast extract, magnesium sulphate, sodium nitrate, Tween 80, and waste office paper), physical factors (temperature, pH and time) and biological factor (inoculum size) were examined using two level full factorial design to check the key factors significantly affecting the cellulase production. The central composite design (CCD) was used to optimize the vital fermentation parameters, such as carbon (WOP), nitrogen, pH, and inoculum concentration in the medium for achieving higher cellulase production. The optimum medium composition was found to be WOP (9â¯g/L), sodium nitrate (0.35â¯g/L), inoculum size (6.56%) and pH 4.72. The model prediction of 2.46â¯U/mL cellulase activity at optimum conditions was verified experimentally as 2.42â¯U/mL.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Waste Management - Volume 79, September 2018, Pages 491-500
Journal: Waste Management - Volume 79, September 2018, Pages 491-500
نویسندگان
Anu Sadasivan Nair, Huda Al-Battashi, Ahlam Al-Akzawi, Neelamegam Annamalai, Ashish Gujarathi, Saif Al-Bahry, Gurpreet Singh Dhillon, Nallusamy Sivakumar,