کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11033789 | 1569758 | 2018 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparative transcriptome analysis of genes involved in Na+ transport in the leaves of halophyte Halogeton glomeratus
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کلمات کلیدی
qRT-PCRKEGGVacuolar pyrophosphataseQuantitative reverse transcriptase-PCRV-PPaseTMDSOS1PacbioCoGNGSROS - ROSTranscriptome - ترانسکریپتومNext-generation sequencing - تعیین توالی نسل بعدیfull-length - تمام طولSalt stress - تنش شوریConsensus sequence - توالی انضباطclusters of orthologous groups - خوشه های گروه های ارتوقیTransmembrane domains - دامنه های ترانزیستورKyoto Encyclopaedia of Genes and Genomes - دایره المعارف کیوتو ژن ها و ژنوم هاDEIS - دیسPacific Biosciences - علوم بیولوژیکی اقیانوس آرامHalophyte - هالوفیGene ontology - هستیشناسی ژنیCdS - کادمیم سولفید، سولفید کادمیمCoding DNA Sequence - کدگذاری دنباله DNAReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Compartmentalization of Na+ into vacuoles is considered to be the most critical aspect of salt tolerance in H. glomeratus, an annual, succulent halophyte. Previous analysis of transcriptome involved in the H. glomeratus salt stress response relied on next-generation sequencing technologies that limit the capture of accurately spliced, full-length isoforms. To gain deeper insights into its salt stress response, we used the H. glomeratus Iso-Seq transcriptome database as a reference, and subsequent next-generation sequencing was subjected to various NaCl concentrations of leaves from plants revealed 115 upregulated and 87 downregulated differentially expressed isoforms (core DEIs). The majority of the core DEIs were involved in carbohydrate metabolism and energy production and conversion. In contrast, levels of known isoforms encoding Na+ transporters did not change significantly under salt stress. However, 16 core DEIs of unknown function were predicted to possess transmembrane domains, suggesting that these candidate isoforms could be involved in Na+ transport in H. glomeratus. These results suggest a potential means for identification of novel Na+ transporters, in addition to providing a foundation for further investigation of Na+ transport networks in halophytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 678, 15 December 2018, Pages 407-416
Journal: Gene - Volume 678, 15 December 2018, Pages 407-416
نویسندگان
Juncheng Wang, Baochun Li, Lirong Yao, Yaxiong Meng, Xiaole Ma, Yong Lai, Erjing Si, Panrong Ren, Ke Yang, Xunwu Shang, Huajun Wang,