ProTSAV: A protein tertiary structure analysis and validation server

• ProTSAV delivers an effective combination of modules and outclasses their discrete accuracies.
• Analysis from all available resources results in better insights into input protein structure.
• Its high sensitivity and specificity for model structures assure reliable and accurate quality assessment.
• User is furnished with an easy to interpret result in the form of a graphical representation.

Quality assessment of predicted model structures of proteins is as important as the protein tertiary structure prediction. A highly efficient quality assessment of predicted model structures directs further research on function. Here we present a new server ProTSAV, capable of evaluating predicted model structures based on some popular online servers and standalone tools. ProTSAV furnishes the user with a single quality score in case of individual protein structure along with a graphical representation and ranking in case of multiple protein structure assessment. The server is validated on ~ 64,446 protein structures including experimental structures from RCSB and predicted model structures for CASP targets and from public decoy sets. ProTSAV succeeds in predicting quality of protein structures with a specificity of 100% and a sensitivity of 98% on experimentally solved structures and achieves a specificity of 88%and a sensitivity of 91% on predicted protein structures of CASP11 targets under 2 Å.The server overcomes the limitations of any single server/method and is seen to be robust in helping in quality assessment.ProTSAV is freely available at http://www.scfbio-iitd.res.in/software/proteomics/protsav.jsp

Figure optionsDownload high-quality image (416 K)Download as PowerPoint slideProTSAV brings an efficient unification of available resources for quality assessment and outperforms their individual accuracies. By means of ProTSAV, a reliable and accurate quality assessment of model structures for applicability in different fields is facilitated. It furnishes the user with an easy to interpret result in the form of a graphical representation.