کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1178233 1491454 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bradavidin II from Bradyrhizobium japonicum: A new avidin-like biotin-binding protein
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Bradavidin II from Bradyrhizobium japonicum: A new avidin-like biotin-binding protein
چکیده انگلیسی

A gene encoding an avidin-like protein was discovered in the genome of B. japonicum. The gene was cloned to an expression vector and a protein, named bradavidin II, was produced in E. coli. Bradavidin II has an identity of 20–30% and a similarity of 30–40% with previously discovered bradavidin and other avidin-like proteins. It has biochemical characteristics close to those of avidin and streptavidin and binds biotin tightly. In contrast to other tetrameric avidin-like proteins studied to date, bradavidin II has no tryptophan analogous to the W110 in avidin (W120 in streptavidin), thought to be one of the most essential residues for tight biotin-binding. Homology modeling suggests that a proline residue may function analogously to tryptophan in this particular position. Structural elements of bradavidin II such as an interface residue pattern or biotin contact residues could be used as such or transferred to engineered avidin forms to improve or create new tools for biotechnological applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1784, Issues 7–8, July–August 2008, Pages 1002–1010
نویسندگان
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