کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1178407 1491449 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Glycogen is the primary source of glucose during the lag phase of E. coli proliferation
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Glycogen is the primary source of glucose during the lag phase of E. coli proliferation
چکیده انگلیسی

In the studies of Escherichia coli (E. coli), metabolomics analyses have mainly been performed using steady state culture. However, to analyze the dynamic changes in cellular metabolism, we performed a profiling of concentration of metabolites by using batch culture. As a first step, we focused on glucose uptake and the behavior of the first metabolite, G6P (glucose-6-phosphate). A computational formula was derived to express the glucose uptake rate by a single cell from two kinds of experimental data, extracellular glucose concentration and cell growth, being simulated by Cell Illustrator. In addition, average concentration of G6P has been measured by CE-MS. The existence of another carbon source was suggested from the computational result. After careful comparison between cell growth, G6P concentration, and the computationally obtained curve of glucose uptake rate, we predicted the consumption of glycogen in lag phase and its accumulation as an energy source in an E. coli cell for the next proliferation. We confirmed our prediction experimentally. This behavior indicates the importance of glycogen participation in the lag phase for the growth of E. coli. This article is part of a Special Issue entitled: Computational Methods for Protein Interaction and Structural Prediction.

Figure optionsDownload high-quality image (245 K)Download as PowerPoint slideHighlights
► Glycogen acts as the main sugar source in the lag phase of E. coli proliferation.
► Glycogen starts its accumulation from the onset of the log phase.
► G6P concentration has two peaks, caused by intracellular glycogen and extracellular glucose.
► A computational model of carbohydrate metabolism around G6P was constructed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1824, Issue 12, December 2012, Pages 1442–1448
نویسندگان
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