Comparative proteomic analysis of malignant pleural mesothelioma: Focusing on the biphasic subtype

• We performed a comparative proteomic analysis of biphasic pleural mesothelioma (BPM) by 2D/MS approach.
• Twenty-two proteins were found differentially expressed in significant manner.
• In B-PM the peculiarity of expression of serum amyloid A1 and gamma enolase was confirmed by western blot analysis.
• Heat map obtained by Ingenuity software analysis suggests a profile of activation typical of B-PM.

Malignant pleural mesothelioma (MPM) is a rare cancer originated from pleural mesothelial cells. MPM has been associated with long-term exposure to asbestos. In this work we performed a comparative proteomic analysis of biphasic pleural mesothelioma (B-PM).Tissue biopsies were obtained from 61 patients who were subjected to a diagnostic thoracoscopy. 2D/MS based approach was used for proteomic analysis. The 22 proteins found differentially expressed in B-PM, with respect to benign, were analyzed by Ingenuity Pathways Analysis and compared with those obtained for epitheliod pleural mesothelioma (E-PM). A different activation of transcription factors, proteins and cytokines were observed between two subtypes.

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