A simple protocol for venom peptide barcoding in scorpions

• Scorpion venom contains many species-specific peptides which target ion channels in cell membranes.
• Venom peptides of single scorpions are detectable by MALDI-TOF MS which makes this technique suitable for peptide barcoding.
• We describe a very simple protocol for venom extraction that facilitates reproducibility.

Scorpion venoms contain many species-specific peptides which target ion channels in cell membranes. Without harming the scorpions, these peptides can easily be extracted and detected by MALDI-TOF mass spectrometry. So far, only few studies compared the venom of different species solely for taxonomic purposes. Here, we describe a very simple protocol for venom extraction and mass fingerprinting that was developed for peptide barcoding (venom code for species identification) and facilitates reproducibility if sample preparation is performed under field conditions. This approach may serve as suitable basis for a taxonomy-oriented scorpion toxin database that interacts with MALDI-TOF mass spectra.

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