Inflammatory markers in Huntington's disease plasma—A robust nanoLC–MRM-MS assay development

• An MRM assay was developed for quantification of complement components and CRP in plasma.
• A robust nanoLC–MRM-MS assay platform is presented.
• The linear range of the assay is at least 4 orders of magnitude with LOQs in the attomolar range.
• The assay was successfully utilized for the analysis of an HD patient cohort.

The development of an MRM assay for the measurements of six inflammatory markers is presented. We report a robust and sensitive quantitative assay with a relative standard deviation of <15% that accounts for the entire sample processing. The assay has a dynamic range with 4 orders of magnitude and the LOQs are in the attomolar range. We used plasma from Huntington's disease gene carriers and healthy controls to compare our MRM method with antibody based methods. Importantly, we found a good agreement between assays for the measurement of C-reactive protein, in contrast to complement component 3 and complement factor H.

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