Proteomic insights into non-small cell lung cancer: New ideas for cancer diagnosis and therapy from a functional viewpoint

• Combined 2D-DIGE analysis of two different lung cancer subtypes (SCC and AC) and cultured cells.
• Specific analysis of the Sec62 interactome by co- immunoprecipitation.
• Plastin-3 and Vimentin are differentially abundant in all proteomic screens.
• The results hint toward a Sec62- and Ca2+-dependent actin remodeling.
• This suggests an explanation for Sec62's influence on tumor cell migration.

We recently characterized SEC62 as an oncogene in non-small-cell lung cancer (NSCLC). Here we aimed to gain further insight into the molecular mechanisms of the cancer-related functions of this oncogene. We performed 2D-DIGE proteome analysis of tumor material from patients with NSCLC and of HEK293 cells stably overexpressing plasmid-encoded SEC62, combined with investigation of the Sec62 interactome. Furthermore, we analyzed the proteomic effects of siRNA-mediated depletion of the Sec62-interacting protein Sec63. We identified a comprehensive list of differentially regulated proteins, providing new insights into the molecular mechanisms of the cancer-related functions of Sec62 in cell migration, drug resistance, and Ca2+-homeostasis.

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