کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1184016 | 1491808 | 2013 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Laser microdissection and two-dimensional difference gel electrophoresis with alkaline isoelectric point immobiline gel reveals proteomic intra-tumor heterogeneity in colorectal cancer
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To approach heterogeneity of tumor tissues, we examined proteomes of normal and tumor tissues from colorectal cancer patients by laser microdissection and 2D-DIGE with saturation dye and alkaline range IPG gel. By distinguishing the tumor cells based on tissue localization, we found that a range of protein spots showed different intensity between normal and diseased tissues. Subsequent mass spectrometric protein identification identified the proteins for protein spots. We found that the protein spots from identical proteins showed the different tissue localization in colorectal cancer. These results highlight the potential utilities of laser microdissection, 2D-DIGE, and immobilized pH gradient gel with alkaline range.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: EuPA Open Proteomics - Volume 1, 2013, Pages 17–29
Journal: EuPA Open Proteomics - Volume 1, 2013, Pages 17–29
نویسندگان
Hirotaka Yonemori, Daisuke Kubota, Hirokazu Taniguchi, Hitoshi Tsuda, Shin Fujita, Yasufumi Murakami, Tadashi Kondo,