کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1184435 1491801 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A quality control of proteomic experiments based on multiple isotopologous internal standards
ترجمه فارسی عنوان
کنترل کیفی آزمایش های پروتئومیک بر اساس استانداردهای داخلی چندین ایزوتوپولوژیک
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Toward harmonization of proteomic data.
• Simple protocol for internal quality control.
• Use of multiple variants of isotopically labeled peptides.

The harmonization of proteomics experiments facilitates the exchange and comparison of results. The definition of standards and metrics ensures reliable and consistent data quality. An internal quality control procedure was developed to assess the different steps of a proteomic analysis workflow and perform a system suitability test. The method relies on a straightforward protocol using a simple mixture of exogenous proteins, and the sequential addition of two sets of isotopically labeled peptides added to reference samples. This internal quality control procedure was applied to plasma samples to demonstrate its easy implementation, which makes it generic for most proteomics applications.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: EuPA Open Proteomics - Volume 8, September 2015, Pages 16–21
نویسندگان
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