کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1184440 | 1491801 | 2015 | 7 صفحه PDF | دانلود رایگان |
• Long time storage (archiving) of biological specimens on MALDI MS targets.
• Nano-coated polymer-based microscope format MALDI MS target.
• One-way and cheap MALDI MS target.
• Storage of peptides and proteins in the MALDI MS matrix for six months.
• Reanalysis of partly used MALDI MS sample preparations.
Archiving of biological specimens is important due to the importance of reanalysis of already prepared MALDI MS samples or simultaneuous analysis of a sample series. Proteins/peptides and digests were prepared for measurement on a polymer-based metal nano-coated MALDI target and subjected to various storage conditions (−80 °C to RT) in a vacuum-sealed pouch and at atmosphere for 6 months. The MS data gathered from these preparations illustrate trends in the aging of different samples and to find optimal storage conditions (−20 or −80 °C in low oxygen environment). The disposable/low cost target proved to be a suitable platform for storage and MALDI MS.
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Journal: EuPA Open Proteomics - Volume 8, September 2015, Pages 48–54