کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1185028 1492080 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of proteolysis on the sialic acid content and bifidogenic activity of ovomucin hydrolysates
ترجمه فارسی عنوان
اثر پروتئولیز بر مقدار اسید سیالیک و فعالیت بیفیدوژنیک هیدرولیز اومومین
کلمات کلیدی
هیدرولیز Ovomucin ؛ مشخصه؛ اسید سیالیک؛ آلرژن بودن؛ فعالیت Bifidogenic
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• The effect of proteolysis of ovomucin on sialic acid contents of hydrolysates were evaluated.
• The bifidogenic activity of ovomucin hydrolysates was tested by Bifidobacterium spp. in vitro.
• An efficient method was applied to hydrolyze ovomucin and all ovomucin hydrolysates were well characterized.
• Allergenicity of all ovomucin hydrolysates was significantly reduced in comparison to ovomucin extracts.
• This work may provide new evidence to use ovomucin hydrolysates as value added ingredients in functional foods.

Ovomucin, accounting for ∼3.5% of egg white proteins, contains 2.6–7.4% of sialic acid; sialic acid is suggested to play important roles in host-recognition, cognition and memory development. However, ovomucin’s limited water solubility might restrict its future applications. The objective of the study was to examine the effect of proteolysis of ovomucin on the sialic acid content and bifidogenic activity of ovomucin hydrolysates. Ovomucin extract was hydrolyzed by 14 proteases with yields and DHs ranging from 42.6% (flavourzyme) to 97.4% (protease N), and 2.4% (flavourzyme) to 46.3% (pronase), respectively. Ovomucin hydrolyzed by pronase and protex 26L showed molecular weight (Mw) distributions less than 40 kDa while others larger than 200 kDa. Allergenicity of ovomucin hydrolysates was significantly reduced (P < 0.05) in comparison to ovomucin extract. The content of sialic acid in hydrolysates ranged from 0.1% (protex 26L) to 3.7% (pronase). Ovomucin hydrolysates did not generally support growth of Bifidobacterium spp. in vitro.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 212, 1 December 2016, Pages 78–86
نویسندگان
, , , ,