Isolation, identification, and antioxidant activity of anthocyanin compounds in Camarosa strawberry

This paper explores the bioactive composition of strawberry (Camarosa variety) [Fragaria × ananassa (Rosaceae Family)] describing its anthocyanin composition and measuring the antioxidant activity (AA) of isolated pigments.Pelargonidin-3-glucoside was the major compound followed by pelargonidin-3-rutinoside and 11 pelargonidin and cyanidin derivatives, determined by LC–DAD–MS. Additionally, delphinidin-3-glucoside, peonidin-3-glucoside, and cyanidin-3-galactoside were tentatively identified for the first time in strawberry. Another original contribution was the identification of 5-carboxypyranopelargonidin-3-glucoside in the Camarosa strawberry variety.We isolated two different fractions with pelargonidin-3-glucoside and pelargonidin-3-rutinoside at 90% and 92% purity, respectively, using CCC. ORAC and FRAP assays showed that pelargonidin-3-glucoside is more active than 3-rutinoside. Thus, the sugar substituent determines AA. Pelargonidin-3-glucoside contributed to 32.61% (ORAC) and 17.58% (FRAP) of the overall AA of the extract.We submitted both the strawberry and the purified anthocyanin fractions to a simulated digestion process. The pelargonidin-3-rutinoside fraction and polymeric compounds maintained their AA.