کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1186106 963426 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of components in culture medium on glutamate decarboxylase activity and γ-aminobutyric acid accumulation in foxtail millet (Setaria italica L.) during germination
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Effects of components in culture medium on glutamate decarboxylase activity and γ-aminobutyric acid accumulation in foxtail millet (Setaria italica L.) during germination
چکیده انگلیسی

The effects of glutamic acid (Glu), pyridoxal-5-phosphate (PLP) and calcium chloride (CaCl2) in culture medium on glutamate decarboxylase (GAD) activity and γ-aminobutyric acid (GABA) accumulation in foxtail millet (Setaria italica L.) during germination were investigated in this study. The components in culture medium for GABA accumulation were optimised using response surface methodology (RSM). Results showed that GAD activity and GABA yield were dependent on the addition of Glu, PLP and CaCl2 into the culture medium. Box–Behnken design indicated that the optimal culture components for GABA accumulation were: Glu at a concentration of 1.2 mg/ml, PLP at a concentration of 50 μM and CaCl2 at a concentration of 2.5 mM. Under the optimal conditions, the maximal production of GABA (42.9 mg/100 g FW) was obtained. Analysis of variance for the regression model suggested that the model can quite exactly predict GABA accumulation in millet during germination.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 116, Issue 1, 1 September 2009, Pages 152–157
نویسندگان
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