کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1186733 | 963446 | 2010 | 6 صفحه PDF | دانلود رایگان |
An anti-DON monoclonal antibody (Mab) was produced from a stable hybridoma cell line, 12D1, generated by the fusion of SP2/O myeloma cells with spleen cells isolated from a Balb/c mouse immunized with DON–cationic bovine serum albumin (CBSA) conjugate. The 12D1 Mab belongs to the immunoglobulin G1 (κ-chain) isotype.A colloidal gold immunochromatographic strip (ICS) test for rapid detection of deoxynivalenol (DON) in wheat and maize samples was also developed using this Mab. The ICS test, which has a detection limit of 50 ng mL−1 for DON and can be completed in 10 min. Analysis of DON in wheat and maize samples revealed that data obtained from ICS test were in a good agreement with those obtained from ELISA and GC/MS. The results demonstrate that the ICS test can be used as qualitative tool for screening technique of DON on-site.
Journal: Food Chemistry - Volume 119, Issue 2, 15 March 2010, Pages 834–839