کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1187049 963454 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison of two different methods for the separation of lipid classes and fatty acid methylation in reindeer and fish muscle
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Comparison of two different methods for the separation of lipid classes and fatty acid methylation in reindeer and fish muscle
چکیده انگلیسی

In the present study different methods for the separation of lipid classes and methylation were compared and evaluated for method optimisation in muscle tissue. Fish and reindeer muscle were tested as fish represents a high proportion of polyunsaturated FA (PUFA) and reindeer a moderate amount of PUFA. Lipids were separated either by solid phase extraction (SPE) into neutral (NL) and polar lipids (PL) or by thin layer chromatography into triacylglycerols (TAG) and phospholipids. All fractions were methylated with or without boron trifluoride (BF3). The two different separation methods showed differences between PL and phospholipids, while TAG and NL were largely similar. Methylation method did not lead to important differences when comparing within the different separation methods, though methylation with BF3 generally resulted in similar or higher values of methylated FA, indicating a more complete methylation. The content of PUFA in the muscle tissue did not affect these outcomes. Separation of lipids by SPE, followed by methylation with BF3 was judged to be the best overall method, though not suitable for analysis of samples with labile fatty acids.


► Separation method showed significant effects for PL and phospholipids.
► Methylation method did not cause differences within the separation methods.
► Methylation with BF3 indicated a more complete methylation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 128, Issue 3, 1 October 2011, Pages 811–819
نویسندگان
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