کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1187406 | 963462 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Assessment and validation of methods for the determination of γ-glutamyltransferase activity in sheep milk
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The determination of enzymatic activity in sheep milk is still today a practically unexplored field of research. This study proposes and fully validates two analytical procedures (i.e. by means of UV-vis spectrophotometry and RP-HPLC methods) for the determination of γ-glutamyltransferase activity in sheep milk. Both methods are characterised by low detection and quantification limits, excellent linearity over a wide enzymatic activity interval, very good repeatability and reproducibility, and are bias-free. The RP-HPLC method provides better sensitivity and automation capability levels than that of UV-vis, and its use is hence suggested for screening purposes. These methods have been preliminarily tested with a number of real samples of whole sheep milk, obtaining an average γ-glutamyltransferase activity value of 2.93 ± 0.50 U mlâ1 and a range from 2.72 ± 0.10 U mlâ1 to 3.46 ± 0.11 U mlâ1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 115, Issue 4, 15 August 2009, Pages 1519-1523
Journal: Food Chemistry - Volume 115, Issue 4, 15 August 2009, Pages 1519-1523
نویسندگان
Carlo Piga, Pietro Paolo Urgeghe, Giovanni Piredda, Maria Francesca Scintu, Gavino Sanna,