کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1188475 963489 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparative separation of flavonoids in Adinandranitida leaves by high-speed counter-current chromatography and their effects on human epidermal carcinoma cancer cells
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Preparative separation of flavonoids in Adinandranitida leaves by high-speed counter-current chromatography and their effects on human epidermal carcinoma cancer cells
چکیده انگلیسی

Preparative separation of camellianins A and B in Adinandra nitida leaves, which is a flavonoid-rich plant source with great practical prospects, was conducted by high-speed counter-current chromatography (HSCCC) with a solvent system composed of ethyl acetate–ethanol–water (5:1:5, v/v). Apigenin (their aglycone) was prepared by hydrolysing a water extract and recrystallising. The purity and identity of these flavonoids was confirmed by HPLC-ESI/MS, and 1H and 13C NMR. These flavones were used in cultures of the A431 cell line. Their inhibitory effect was evaluated by using the MTT assay. The results showed that three flavones could significantly inhibit the growth of human epidermal carcinoma cancer cells in a concentration-dependent manner. After 48 h of treatment, IC50 values of camellianin A, camellianin B and apigenin against A431 tumour cells were 9.09 μM, 12.5 μM, 21.0 μM, respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 115, Issue 3, 1 August 2009, Pages 1158–1163
نویسندگان
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