In vitro study of antioxidant capacity and antibacterial activity on oral anaerobes of a grape seed extract

Grape seeds are considered rich sources of polyphenolic compounds that show antioxidant or antimicrobial effects. The aim of the present work was to investigate the effect of a grape seed extract (GSE) on two oral anaerobes closely associated with periodontal diseases and its antioxidant action.The antimicrobial activity was determined using the macro dilution broth technique and also tested on a multi-species biofilm grown on saliva-coated hydroxyapatite discs. The evaluation of antioxidant activity was based on the capacity of a sample to scavenge the ABTS radical cation as compared to a standard antioxidant (Trolox). GSE had a bacteriostatic effect on the anaerobes. At a concentration of 2000 μg/ml, it significantly decreased the formation of biofilm. High TEAC values were registered and this extract exhibited greater antioxidant capacity than vitamins C and E.These findings indicated that GSE could be used in oral hygiene for the prevention of periodontitis.