کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1188984 | 963502 | 2009 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A novel quantum dot-based fluoroimmunoassay method for detection of Enrofloxacin residue in chicken muscle tissue A novel quantum dot-based fluoroimmunoassay method for detection of Enrofloxacin residue in chicken muscle tissue](/preview/png/1188984.png)
A rapid indirect competitive fluorescence-linked immunosorbent assay (cFLISA) based on quantum dots (QDs) as the fluorescent marker has been developed for the detection of Enrofloxacin (ENR) in chicken muscle tissue. The end-point fluorescent detection system was carried out using QDs conjugated with goat anti-mouse secondary antibody. The cFLISA method allowed for ENR determination in a liner working range of 1–100 ng mL−1 with the 50% inhibition value (IC50) of 8.3 ng mL−1 and the limit of detection (LOD) of 2.5 ng mL−1. The recoveries for chicken muscle samples spiked with ENR at levels of 50–200 μg kg−1 ranged from 81% to 94% with coefficients of variation (CV) of 10–13%. In real chicken tissue sample analysis, the results of cFLISA were similar to those obtained from an indirect competitive enzyme-linked immunosorbent assay (cELISA) to a high performance liquid chromatography method (HPLC), which indicated that cFLISA is suitable as screening method for the monitoring of veterinary drug residues.
Journal: Food Chemistry - Volume 113, Issue 4, 15 April 2009, Pages 1197–1201