کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1189374 963510 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification and characterization of polyphenol oxidase from Ferula sp.
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Purification and characterization of polyphenol oxidase from Ferula sp.
چکیده انگلیسی

Polyphenol oxidase (PPO) of several Ferula sp. was extracted and purified through (NH4)2SO4 precipitation, dialysis, and gel filtration chromatography. Leaf and stem extracts were used for the determination of enzyme properties. Optimum conditions, for pH, temperature, and ionic strength were determined. The best substrates of PPO were catechol for leaf and (−) epicatechin for stem samples. Optimum pH and temperature were determined. KM and Vmax values were 2.34 × 10−3 M and 8541 EU/ml for catechol, and 2.89 × 10−3 M and 5308 EU/ml for (−) epicatechin. The most effective inhibitor was sodium diethyl dithiocarbamate for leaf samples and sodium metabisulphite for stem samples. Both inhibitors indicated competitive reactions. PPO showed irreversible denaturation after 40 min at 60 °C.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 95, Issue 3, April 2006, Pages 503–508
نویسندگان
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