Carbon-14 biolabeling of flavanols and chlorogenic acids in Crataegus monogyna cell suspension cultures

Pure radiolabeled phenolic compounds (including catechins, procyanidins and chlorogenic acids) were obtained by administrating 14C-l-phenylalanine to Crataegus monogyna cell suspension cultures. After a simple process of purification, pure compounds with high specific activities were recovered. In the present study, we describe a suitable method for obtaining labeled phenolics, which will be useful tools for in vivo feeding trials to trace their metabolic fate when consumed by animals and for in vitro activity mechanism studies.