کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1211779 1494030 2016 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of oxygenated metabolites of ginsenoside Rg1 in plasma and urine of rat
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Characterization of oxygenated metabolites of ginsenoside Rg1 in plasma and urine of rat
چکیده انگلیسی


• Nine oxygenated metabolites of ginsenoside Rg1 in plasma and urine of rat following i.v. dose were characterized based on the mass spectroscopic data and comparison with chemically synthesized authentic compounds.
• This study represents the first report on the characterization of exact chemical structures of oxygenated metabolites of PPT-type primary ginsenosides formed in vivo.
• We revealed that these oxygenated metabolites were not derived from P450 oxidation or blood oxidation, but were generated from oxidation occurred in rat tissues.

This study describes the characterization of oxygenated metabolites of ginsenoside Rg1 in rat urine and plasma. These in vivo metabolites were profiled by using UHPLC-QTOF MS-based method. On the basis of high-resolution MS/MS data, and comparison with chemically synthesized authentic compounds, nine oxygenated metabolites of Rg1 were characterized as vinaginsenosides 21 and 22 (M1 and M2), vinaginsenoside R15 (M3), 6-O-(β-d-glucopyranosyl)-20-O-(β-d-glucopyranosyl) 3β, 6α, 12β, 20(S)-tetrahydroxy-24ξ-hydroxydammar-25-ene (M4 and M5), floralginsenoside A (M7 and M8), floralginsenoside B (M9) and epoxyginsenoside Rg1 (M13), respectively. Among these metabolites, M4, M5 and M13 are new ginsenosides and others were detected as in vivo metabolites of Rg1 for the first time. In addition, a series of oxygenated metabolites of Rh1 and deglycosylated metabolite of Rg1, were observed and characterized by comparing with compounds synthesized by us, which revealed an association between C-20 configuration and the extent of oxidation metabolism. Appearance of all these metabolites in blood stream and urine after i.v. dosing and oral administration of Rg1 was further examined, which clearly showed that mono-oxygenated metabolites of Rg1 were major circulating metabolites at the early stage after dosing. Characterization of exact chemical structures of these circulating metabolites contribute greatly to our understanding of chemical exposure after consumption of ginseng products, and provide valuable information for explaining multiple bioactivities of ginseng products.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1026, 15 July 2016, Pages 75–86
نویسندگان
, , , , , , , , ,