کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1211860 | 1494024 | 2016 | 6 صفحه PDF | دانلود رایگان |

• A novel UPLC–MS/MS method was developed for the determination of lomefloxacin in rabbit aqueous humor.
• This is the first report of the pharmacokinetics of lomefloxacin using a validated UPLC–MS/MS method.
• The developed method was successfully applied to the pharmacokinetic study of lomefloxacin hydrochloride in rabbit aqueous humor.
• A formulation of lomefloxacin hydrochloride eye drops was designed.
Lomefloxacin is a kind of synthetic fluoroquinolone antibiotic, which is used for the treatment of infectious diseases. In this study, a rapid and efficient liquid chromatography–tandem mass spectrometric assay was developed to determine the concentration of lomefloxacin in rabbit aqueous humor quantitatively. Aqueous humor samples were extracted by protein precipitation. Ofloxacin was chosen as internal standard. The chromatographic separation was achieved on a Kinetex C18 (50 mm × 2.10 mm, 2.6 μm, Phenomenex Corp, USA) column, with a gradient of methanol (0.1% formic acid) and water (0.1% formic acid). Multiple reaction monitoring (MRM) with positive ionization mode was used for the mass analysis.The validation of this method was based on the European Medicines Agency (2011) [1] and US FDA Guidelines (2001) [2]. The calibration range of aqueous humor samples was 5–1200 ng/mL with r = 0.9990 (n = 6). For all QC samples, Inter-and intra-run precisions were less than 15% and accuracies were between 80%–120%. In conclusion, the assay was rapid, sensitive and able to determinate the lomefloxacin in rabbit aqueous humor accurately. At the same time, this method was successfully applied to study the pharmacokinetics of lomefloxacin hydrochloride eye drops and lomefloxacin hydrochloride ophthalmic gel in rabbit aqueous humor.
Journal: Journal of Chromatography B - Volumes 1033–1034, 15 October 2016, Pages 187–192