Determination of the oxidative stress biomarker urinary 8-hydroxy-2′-deoxyguanosine by automated on-line in-tube solid-phase microextraction coupled with liquid chromatography–tandem mass spectrometry

• On-line in-tube solid-phase microextraction (SPME) of 8-hydroxy-2′-deoxyguanosine (8-OHdG) was optimized.
• 8-OHdG was determined by liquid chromatography–tandem mass spectrometry (LC–MS/MS).
• In-tube SPME coupled with LC–MS/MS method is automated, simple, rapid, selective and sensitive.
• 8-OHdG could be directly and quantitatively analyzed from human urine sample without any other pre-treatment.
• This method is a useful tool for assessing oxidative stress and the effects of antioxidant intake.

A simple and sensitive method for the determination of 8-hydroxy-2′-deoxyguanosine (8-OHdG), a marker of oxidative DNA damage in human urine, was developed using automated on-line in-tube solid-phase microextraction (SPME) coupled with stable isotope-dilution liquid chromatography–tandem mass spectrometry (LC–MS/MS). Creatinine was also analyzed simultaneously to normalize urine volume by the in-tube SPME LC–MS/MS method, and 8-OHdG and creatinine were separated within 3 min using a Zorbax Eclipse XDB-C8 column. Electrospray MS/MS for these compounds was performed on an API 4000 triple quadruple mass spectrometer in the positive ion mode by multiple reaction monitoring. The optimum in-tube SPME conditions were 20 draw/eject cycles of 40 μL of sample at a flow rate of 200 μL/min using a Carboxen 1006 PLOT capillary column as an extraction device. The extracted compounds were easily desorbed from the capillary by passage of the mobile phase, and no carryover was observed. The calibration curve for 8-OHdG using its stable isotope-labeled internal standard was linear in the range of 0.05–10 ng/mL, and the detection limit was 8.3 pg/mL. The intra-day and inter-day precision (relative standard deviations) were below 3.1% and 9.6% (n = 5), respectively. This method was applied successfully to the analysis of urine samples without any other pretreatment and interference peaks, with good recovery rates above 91% in spiked urine samples. The limits of quantification of 8-OHdG and creatinine in 0.1 mL urine samples were about 0.32 and 0.69 ng/mL (S/N = 10), respectively. This method was utilized to assess the effects of smoking, green tea drinking and alcohol drinking on the urinary excretion of 8-OHdG.

8-Hydroxy-2′-deoxyguanosine (8-OHdG) is a marker of oxidative DNA damage in human urine. 8-OHdG can be simultaneously analyzed with creatinine to normalize urine volume by automated on-line in-tube solid-phase microextraction (SPME) coupled with stable isotope-dilution liquid chromatography–tandem mass spectrometry (LC–MS/MS).Figure optionsDownload as PowerPoint slide