کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1211930 1494034 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Antibodies as means for selective mass spectrometry
ترجمه فارسی عنوان
آنتیبادی ها به عنوان وسیله ای برای طیف سنجی جرمی انتخابی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• The combination of affinity enrichment and mass spectrometry is reviewed.
• This combination results in a gain in both sensitivity and specificity.
• Developed technologies for protein analysis and quantification are discussed.
• This is a growing area of research with the aim to enter the clinic.

For protein analysis of biological samples, two major strategies are used today; mass spectrometry (MS) and antibody-based methods. Each strategy offers advantages and drawbacks. However, combining the two using an immunoenrichment step with MS analysis brings together the benefits of each method resulting in increased sensitivity, faster analysis and possibility of higher degrees of multiplexing. The immunoenrichment can be performed either on protein or peptide level and quantification standards can be added in order to enable determination of the absolute protein concentration in the sample. The combination of immunoenrichment and MS holds great promise for the future in both proteomics and clinical diagnostics. This review describes different setups of immunoenrichment coupled to mass spectrometry and how these can be utilized in various applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1021, 15 May 2016, Pages 3–13
نویسندگان
, , ,