کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1212021 | 1494048 | 2015 | 6 صفحه PDF | دانلود رایگان |
• A sensitive LC–MS/MS method was developed to quantify avobenzone in rat biological matrices.
• The assay was validated for rat plasma, stratum corneum, epidermis, and dermis.
• A percutaneous absorption of avobenzone was examined in rats after topical application.
• Avobenzone was found to penetrate into the skin layers but was not absorbed in the circulation.
This study describes the development of a sensitive high-performance liquid chromatography–electrospray ionization-tandem mass spectrometry (LC–ESI-MS/MS) method for the quantification of avobenzone in rat plasma and skin layers. Separations were performed on a Zorbax SB C8 column using a binary gradient mobile phase composed of acetonitrile and 0.1% formic acid in water. The assay achieved LLOQ of 0.5 ng/ml for plasma, 5 ng/ml for stratum corneum, and 10 ng/ml for epidermis and dermis. This method was applied to a percutaneous absorption study of avobenzone in rats. At 12 h following topical application of emulsion and lotion (applied amount of avobenzone 11.7 mg/kg), avobenzone was found primarily in the stratum corneum (16.3–17.8%) followed by epidermis (2.0–3.4%) and dermis (0.11–0.15%). Avobenzone was not quantifiable in the plasma samples collected over a 12 h sampling period. Given the excellent plasma assay sensitivity, this study provides evidence that the systemic absorption of avobenzone is insignificant, if any, after topical application.
Journal: Journal of Chromatography B - Volume 1003, 15 October 2015, Pages 41–46