کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1212208 1494062 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid determination of ruscogenin in rat plasma with application to pharmacokinetic study
ترجمه فارسی عنوان
تعیین سریع راسکوزین در پلاسمای موش صحرایی با استفاده از مطالعات فارماکوکینتیک
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• A method for the analysis of ruscogenin was developed.
• The method produced a linear range of 2–1000 ng/mL ruscogenin in rat plasma.
• The method was successful at detecting ruscogenin from treated rats.

A sensitive and rapid ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine ruscogenin in rat plasma using midazolam as the internal standard (IS). Sample preparation was accomplished through a liquid–liquid extraction procedure with ethyl acetate to 0.2 mL plasma sample. The analyte and IS were separated on an Acquity UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) with the mobile phase of acetonitrile and 1% formic acid in water with gradient elution at a flow rate of 0.40 mL/min. Ruscogenin and IS were eluted at 1.74 and 1.11 min, respectively. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with positive-ion electrospray ionization (ESI) by multiple reactions monitoring (MRM) of the transitions at m/z 431.2 → 287.0 for ruscogenin and m/z 326.2 → 291.1 for IS. The linearity of this method was found to be within the concentration range of 2–1000 ng/mL with a lower limit of quantification of 2 ng/mL. Only 2.0 min was needed for an analytical run. The matrix effect was 92.4–107.3% for ruscogenin. The intra- and inter-day precision (RSD%) were less than 11.2% and accuracy (RE%) was within ±9.8%. The recovery ranged from 75.4% to 86.3%. Ruscogenin was sufficiently stable under all relevant analytical conditions. The method was also successfully applied to the pharmacokinetic study of ruscogenin in rats.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 985, 15 March 2015, Pages 71–74
نویسندگان
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