کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1212369 1494075 2014 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of biomolecular interactions using affinity microcolumns: A review
ترجمه فارسی عنوان
تجزیه و تحلیل متابولیسم های بیولوژیکی با استفاده از میکرولون های هوشیاری: بررسی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• The use of affinity microcolumns for studying biointeractions is discussed.
• The types of affinity microcolumns and their potential uses are described.
• Methods for estimating equilibrium constants and rate constants are considered.
• Several applications are presented, involving both small and large biomolecules.

Affinity chromatography has become an important tool for characterizing biomolecular interactions. The use of affinity microcolumns, which contain immobilized binding agents and have volumes in the mid-to-low microliter range, has received particular attention in recent years. Potential advantages of affinity microcolumns include the many analysis and detection formats that can be used with these columns, as well as the need for only small amounts of supports and immobilized binding agents. This review examines how affinity microcolumns have been used to examine biomolecular interactions. Both capillary-based microcolumns and short microcolumns are considered. The use of affinity microcolumns with zonal elution and frontal analysis methods are discussed. The techniques of peak decay analysis, ultrafast affinity extraction, split-peak analysis, and band-broadening studies are also explored. The principles of these methods are examined and various applications are provided to illustrate the use of these methods with affinity microcolumns. It is shown how these techniques can be utilized to provide information on the binding strength and kinetics of an interaction, as well as on the number and types of binding sites. It is further demonstrated how information on competition or displacement effects can be obtained by these methods.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 968, 1 October 2014, Pages 49–63
نویسندگان
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