کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1212746 1494093 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Method development and validation for simultaneous determination of lumefantrine and its major metabolite, desbutyl lumefantrine in human plasma using RP-HPLC/UV detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Method development and validation for simultaneous determination of lumefantrine and its major metabolite, desbutyl lumefantrine in human plasma using RP-HPLC/UV detection
چکیده انگلیسی


• Developed a rapid method for the analysis of lumefantrine in biological samples.
• This method requires only small volume of low toxicity extraction solvent.
• This approach proved to serve as a simple, inexpensive and eco-friendly method.

A simple, specific, precise and rapid RP-HPLC–UV method was developed for simultaneous determination of lumefantrine and its metabolite desbutyl lumefantrine in human plasma. Experimental parameters were optimized and the method was validated according to standard guidelines. The method showed adequate separation for lumefantrine and desbutyl lumefantrine and best resolution was achieved with Supelco Discovery HS C18 RP (150 mm × 4.6 mm, 5 μm) column using acetonitrile and 0.05% trifluroacetic acid (70:30, v/v) as a mobile phase pumped at a flow rate of 1.0 ml/min and wavelength of 335 nm. The method was linear over the concentration range of 10–12,000 ng/ml. The lower limit of detection (LLOD) and lower limit of quantification (LLOQ) for lumefantrine were 10.0 and 18.0 ng/ml, while for desbutyl lumefantrine were 7.5 and 15.0 ng/ml, respectively. The proposed method was efficiently applied for determination of lumefantrine and desbutyl lumefantrine concentrations in plasma samples for pharmacokinetic studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 944, 1 January 2014, Pages 114–122
نویسندگان
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