کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1212805 1494113 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous quantification of cefpodoxime proxetil and clavulanic acid in human plasma by LC–MS using solid phase extraction with application to pharmacokinetic studies
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Simultaneous quantification of cefpodoxime proxetil and clavulanic acid in human plasma by LC–MS using solid phase extraction with application to pharmacokinetic studies
چکیده انگلیسی

A simple, rapid and selective high performance liquid chromatography–atmospheric pressure chemical ionization-mass spectrometry (HPLC–APCI-MS) method was developed and validated for the simultaneous estimation of cefpodoxime proxetil (CDPX) and clavulanic acid (CA) in human plasma. Extraction of samples was done by solid phase extraction technique (SPE) and chloramphenicol used as internal standard. Chromatographic separation was carried out on a reverse phase Princeton SPHER C18 (150 mm × 4 mm i.d., 5 μm) column using mixture of methanol: acetonitrile: 2 mM ammonium acetate (25:25:50, v/v, pH 3.5) at 0.8 mL/min flow rate. Detection was performed on a single quadrupole MS by selected ion monitoring (SIM) mode via APCI source. The calibration curve was linear within the concentration range, 0.04–4.4 μg/mL and 0.1–10.0 μg/mL for CDPX and CA respectively. Pharmacokinetic parameters of tablet (CDPX 200 mg, CA 125 mg) were evaluated. Cmax, Tmax, T1/2, elimination rate constant (Kel), AUC0–t, and AUC0–∞ of tablet were 2.13 ± 0.06 μg/mL, 2 h, 3.05 ± 0.15 h, 0.24 ± 0.37 h−1, 6.81 ± 0.14 μg h/mL and 7.72 ± 0.23 μg h/mL respectively for cefpodoxime (CP), 5.34 ± 0.28 μg/mL, 2 h, 2.73 ± 0.25 h, 0.26 ± 0.31 h−1, 15.37 ± 0.16 μg h/mL and 16.59 ± 0.53 μg h/mL respectively for CA.


► There was no LC–MS method reported for the simultaneous determination of CP and CA in biological samples.
► We developed and validated a rapid and sensitive LC–MS method for simultaneous estimation of CP and CA in human plasma.
► We evaluated the pharmacokinetic variables of tablet (containing CP 200 mg and CA 125 mg) after a single oral dose administration in twelve healthy human volunteers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 921–922, 15 March 2013, Pages 49–55
نویسندگان
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