کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1212828 | 1494098 | 2013 | 6 صفحه PDF | دانلود رایگان |
• A SPE–HPLC–MS/MS method is established to detect BPA, NP and OP in human urine.
• The exposure levels of BPA, NP and OP in uterine leiomyoma and control group urine sample are investigated.
• There is linkage between the exposure concentration of BPA, NP and OP and uterine leiomyoma disease.
A method was established for the determination of three phenolic environmental estrogens, namely bisphenol A (BPA), nonylphenol (NP) and octylphenol (OP), in urine from women of uterine leiomyoma group (n = 49) and control group (n = 29), by using solid-phase extraction (SPE) coupled with liquid chromatography–tandem mass spectrometry (HPLC–MS/MS). Urine samples were spiked with 2,4,6-tribromophenyl-terminated tetrabromobisphenol-A carbonate oligomer (TBBPA) and nonylphenol D8 (NP-D8) as internal standard (I.S.) and de-conjugated by adding β-glucuronidase and sulfatase before the SPE. The extraction recoveries of BPA, NP and OP were more than 73.3%; the standard curve was linear over the validated concentrations in the range of 1.0–100.0 ng/mL and the limits of detection (LOD) of BPA, NP and OP were 0.32 ng/mL, 0.18 ng/mL and 0.15 ng/mL, respectively. Moreover, by analysing quality control urine samples in 5 days, the results showed that the method was precise and accurate, for the intra- and inter-day CV% within 15.2%. Except that OP was not found (
Journal: Journal of Chromatography B - Volume 938, 1 November 2013, Pages 80–85