کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1212843 1494091 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparative separation and purification of rosmarinic acid from perilla seed meal via combined column chromatography
ترجمه فارسی عنوان
جداسازی آماده سازی و تمیز کردن اسید رزمارینیک از غذای دانه پریلا با استفاده از کروماتوگرافی ستون ترکیبی
کلمات کلیدی
کروماتوگرافی ترکیبی، رزین مکروپوروس، پودر دانه پیمانه، رزین پلی آمید، اسید رومیاریین
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Perilla seed meal is a new source for rosmarinic acid (RA).
• Macroporous and polyamide resins were conducted for RA preparation.
• We optimized the enrichment of RA using different resins.
• RA was obtained through elution on SP825 resin followed by a polyamide resin.

In this study, the preparative separation and purification of rosmarinic acid (RA) from perilla seed meal (PSM), which is a by-product of edible oil production, was achieved using combined column chromatography over macroporous and polyamide resins. To optimize the RA enrichment process, the performance and separation characteristics of nine selected macroporous resins with different chemical and physical properties were investigated. SP825 resin was the most effective: the content of RA increased from 0.27% in the original extract to 16.58% in the 50% ethanol fraction (a 61.4-fold increase). During further purification treatment on polyamide resin, 90.23% pure RA could be obtained in the 70% ethanol fraction. RA with a higher purity (>95%) could also be easily obtained using one crystallization operation. The proposed method is simple, easily operated, cost-effective, and environmentally friendly and is suitable for both large-scale RA production and waste management.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 947–948, 1 February 2014, Pages 41–48
نویسندگان
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