کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1212894 | 1494105 | 2013 | 6 صفحه PDF | دانلود رایگان |

• An imprinted polymer for bisphenol A was designed with hydroquinone as template.
• MIP-SPE method for bisphenol A in milk was developed and validated.
• Testing milk samples, bisphenol A was identified in one at a level of 176 μg kg−1.
• BPA self-association effects were examined with crystallography.
• Pre-polymerization interactions were studied using molecular dynamics simulations.
This paper describes the determination of bisphenol A (BPA) in milk samples, using a novel molecularly imprinted polymer. The imprinted polymer was developed using a rational design approach, and pre-polymerization interactions were investigated using molecular dynamics simulations and X-ray crystallography. A hydroquinone-imprinted polymer was used for solid phase extraction (SPE) clean-up of samples. BPA was quantified by high performance liquid chromatography (HPLC) and fluorescence (FLD) detection. Following validation, the method described was capable of determining bisphenol A in milk down to a limit of detection of 1.32 μg kg−1. The method was applied to a survey (n = 27) of commercial milk products; BPA was detected in one of the samples, at a level of 176 μg kg−1. Test results were confirmed by a parallel UHPLC–MS/MS analytical method. This demonstrates the utility of the hydroquinone-imprinted polymer for application to selective sample clean-up and analysis of bisphenol A in milk, avoiding possible detrimental affects associated with template bleeding and without the need for expensive or difficult-to-obtain template.
Journal: Journal of Chromatography B - Volume 931, 15 July 2013, Pages 164–169