کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1212976 | 1494107 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of antazoline hydrochloride in Beagle dog plasma by HPLC-UV and its application to pharmacokinetics
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determination of antazoline hydrochloride in Beagle dog plasma by HPLC-UV and its application to pharmacokinetics Determination of antazoline hydrochloride in Beagle dog plasma by HPLC-UV and its application to pharmacokinetics](/preview/png/1212976.png)
چکیده انگلیسی
In order to evaluate the pharmacokinetics characteristic of antazoline hydrochloride in Beagle dogs, a sensitive and specific HPLC method was developed and validated using phenacetin as the internal standard (IS). The analyte and the IS were extracted from dog plasma by ethyl acetate under the basic condition. The analyte was separated by a C18 column and detected with a variable wavelength UV-detector. The mobile phase consisted of methanol-5 mmol Lâ1 tetrabutyl ammonium bromide (45:55, v/v) containing 0.5% glacial acetic acid in a flow rate of 1.0 mL minâ1. Standard calibration graph for antazoline was linear over a curve range of 20-1600 ng mLâ1 (R > 0.99) and the lower limit of quantification was 20 ng mLâ1 using a plasma sample of 500 μL. The intra- and inter-day precision values were less than 14.3% relative standard deviation (RSD). The intra-day assay accuracy was in the range of 98.1-100.6% and the inter-day assay accuracy in the range of 99.2-101.1%. The extraction recoveries were on the average of 88.4% for antazoline and 76.8% for IS. Plasma samples were stable at least for 1 month at â20 °C. This method was successfully applied to pharmacokinetics study of antazoline after intravenous administration to Beagle dogs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 929, 15 June 2013, Pages 97-101
Journal: Journal of Chromatography B - Volume 929, 15 June 2013, Pages 97-101
نویسندگان
Xiaotian Li, Yanle Chu, Yu Ke, Linxi Wang, Tong Yu, Lianqi Hao,