کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1213064 1494060 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bioavailability and pharmacokinetics profile of helicid in beagle dogs using gradient elution high performance liquid chromatography electrospray ionization mass spectrometry
ترجمه فارسی عنوان
مشخصات بیوپلاسم و فارماکوکینتیک هلیکید در سگ های گاو با استفاده از روش شستشوی شیب کروماتوگرافی مایع کروماتوگرافی الکترو اسپری اسپکترومتری جرم یونیزاسیون
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• A novel LC–ESI-MS quantitation method was developed.
• The LOD and LLOQ were 0.3 and 1 ng/mL, respectively.
• A random two-period crossover study in 6 dogs was adopted.

A simple, sensitive and reliable gradient elution high performance liquid chromatography electrospray ionization mass spectrometry (HPLC–ESI-MS) method was developed for quantifying helicid in dog plasma. The limit of detection (LOD) and the lower limit of quantitation (LLOQ) were 0.3 and 1 ng/mL, respectively. This method was validated for selectivity, linearity, accuracy and precision, extraction recoveries, matrix effects, carry-over, cross-talk, dilution integrity, stability and incurred sample reanalysis (ISR). Bioavailability and pharmacokinetic parameters of helicid in beagle dogs were researched from a two period crossover design study. After intravenous administration (i.v.), helicid had a mean (±SD) AUC0–∞ of 12062.06 ± 2482.69 ng/mL h and terminal half-life (t1/2z) of 2.91 ± 1.37 h, while Cmax was 35613.23 ± 8157.18 ng/mL. Following intragastric gavage administration (i.g.), AUC0–∞ was 7589.16 ± 1797.20 ng/mL h along with a longer t1/2z of 4.10 ± 4.35 h. Cmax was researched at 0.58 ± 0.20 h. The absolute bioavailability (F) of helicid was 15.74 ± 1.87%.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 988, 15 April 2015, Pages 8–12
نویسندگان
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