کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1213177 | 1494141 | 2012 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Implementation of a cost-effective HPLC/UV-approach for medical routine quantification of donepezil in human serum Implementation of a cost-effective HPLC/UV-approach for medical routine quantification of donepezil in human serum](/preview/png/1213177.png)
A novel, simple, specific and sensitive high performance liquid chromatography (HPLC) assay for the detection and quantification of donepezil in serum of demented patients has been developed and validated. The analytical procedure involves an offline serum preextraction using solid phase extraction (SPE) cartridges (Oasis® HLB, Waters Co). The chromatographic analyses were performed on a Dionex HPLC system with a Phenomenex Luna Phenyl-Hexyl analytical column, and a mobile phase with the two components 0.02 mol/l phosphate buffer and acetonitrile. The flow rate was 0.4 ml/min. For the detection of donepezil three different UV wavelengths were used as an interference-control check. Interference tests between donepezil and 100 of the most commonly used concomitant medications allow quantification of donepezil under the polypharmaceutical conditions of the daily clinical routine. The retention time for donepezil was 12.1 min. The method was validated according to the guidelines of the Society of Toxicology and Forensic Chemistry (GTFCh): The calibration curve was linear over a concentration range from 5 to 160 ng/ml (n = 8/r2 > 0.999). No endogenous compounds were found to interfere with the analyte, which was shown by retention times for the comedication most often prescribed to demented patients. The method had an accuracy of >85%. Intra- and inter-assay coefficients of variation were <6% and <8%, respectively, at three different concentrations. The limit of quantification (LOQ) and the limit of detection (LOD) were found to be 6.1 and 1.7 ng/ml for donepezil. Application of the method to patient serum samples discovered that concentrations suggested as “therapeutic” in the literature may only be reached either by high, off-label dosages or by utilization of inhibitory metabolic effects of the comedication.
► We describe the first assay for TDM of donepezil by HPLC/UV in routine analysis.
► Three different UV detection wavelengths were used to check for peak impurities.
► Most of the dementia patients with donepezil treatment are underdosed.
► Off-label doses will be necessary to reach the therapeutic concentrations.
► Such an approach can be supported by TDM as suggested in this paper.
Journal: Journal of Chromatography B - Volumes 881–882, 15 January 2012, Pages 1–11