A dried blood spots technique based LC-MS/MS method for the analysis of posaconazole in human whole blood samples

► We describe the application of DBS sampling and extraction to screen posaconazole in human whole blood. ► We demonstrate the advantages of our assay for potential use in therapeutic drug monitoring of sick patients. ► We examine the posaconazole DBS method's ruggedness and robustness by implementing guidelines commonly used in FDA regulated bioanalytical environments. ► Blood spot homogeneity was maintained and yielded accurate, precise and reproducible quantitation results.