کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1213474 | 1494136 | 2012 | 8 صفحه PDF | دانلود رایگان |

A highly sensitive and specific liquid chromatography–electrospray ionization tandem mass spectrometric (LC–ESI-MS/MS) method for investigating the in vivo metabolites of almotriptan in rat plasma, feces and urine was developed. Chromatographic separation was achieved on a Lichrospher RP-18 column (250 mm × 4.6 mm, 5 μm), using 20 mM ammonium acetate (pH 3.5) and acetonitrile (60:40, v/v) as a mobile phase at 25 °C. MS/MS detection was performed by positive ion electrospray ionization using target ions at m/z 336 [M+H]+, m/z 368 and m/z 282 [M+H]+ for almotriptan and its two metabolites, respectively. Two metabolites viz., γ-aminobutyric acid and sulfonamide were detected in plasma as well as feces after 24 h of oral administration of almotriptan, while only γ-aminobutyric acid was found in urine. The method was sensitive with a lower limit of quantification of 1.43 ng/mL and linear over the range of 1.43–5000 ng/mL in plasma. The method was validated and successfully applied to a pharmacokinetic study of almotriptan in rat plasma using sumatriptan as an internal standard. The peak plasma concentration (Cmax) after 0.3 h of 5 mg/kg oral dose of almotriptan was determined to be 69.85 ng/mL.
► Metabolite identification has been facilitated in high resolution LC–MS/MS.
► The method was successfully applied to study pharmacokinetics of almotriptan.
► Simple protein precipitation method was used for rat plasma in sample preparation.
► Good separation and resolution were obtained on Lichrospher RP-18 column.
Journal: Journal of Chromatography B - Volumes 891–892, 1 April 2012, Pages 44–51